Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Breast

Cell type

MDA-MB-436

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MB436 cells, H3K9me3 ChIP

cell line

MB436

cell type

Breast cancer cell line

treatment

Control

chip antibody

H3K9me3 (Abcam, ab8898, lot GR237138-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

22812547

Reads aligned (%)

83.8

Duplicates removed (%)

3.9

Number of peaks

1181 (qval < 1E-05)

hg19

Number of total reads

22812547

Reads aligned (%)

82.5

Duplicates removed (%)

4.9

Number of peaks

1119 (qval < 1E-05)