Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Breast

Cell type

MDA-MB-436

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MB436 cells, H3K36me3 ChIP

cell line

MB436

cell type

Breast cancer cell line

treatment

Control

chip antibody

H3K36me3 (Abcam, ab9050, lot GR233723-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

32110010

Reads aligned (%)

92.7

Duplicates removed (%)

3.2

Number of peaks

1453 (qval < 1E-05)

hg19

Number of total reads

32110010

Reads aligned (%)

92.1

Duplicates removed (%)

4.0

Number of peaks

1442 (qval < 1E-05)