Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MB231 cells, H3K4me1 ChIP
cell line
MB231
cell type
Breast cancer cell line
treatment
Control
chip antibody
H3K4me1 (Abcam, ab8895, GR193882-2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
33984215
Reads aligned (%)
88.7
Duplicates removed (%)
3.1
Number of peaks
30852 (qval < 1E-05)

hg19

Number of total reads
33984215
Reads aligned (%)
88.4
Duplicates removed (%)
3.4
Number of peaks
30923 (qval < 1E-05)

Base call quality data from DBCLS SRA