Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Breast
Cell type
UACC-812
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
UACC812 cells, H3K4me3 ChIP
cell line
UACC812
cell type
Breast cancer cell line
treatment
Control
chip antibody
H3K4me3 (Abcam, ab8580, lot GR1902371-1 and GR224370-1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
33087300
Reads aligned (%)
95.0
Duplicates removed (%)
30.1
Number of peaks
28777 (qval < 1E-05)

hg19

Number of total reads
33087300
Reads aligned (%)
94.8
Duplicates removed (%)
30.3
Number of peaks
28706 (qval < 1E-05)

Base call quality data from DBCLS SRA