Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7 cells, input

cell line

MCF7

cell type

Breast cancer cell line

treatment

Control

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

32584047

Reads aligned (%)

90.3

Duplicates removed (%)

1.3

Number of peaks

530 (qval < 1E-05)

hg19

Number of total reads

32584047

Reads aligned (%)

89.4

Duplicates removed (%)

2.5

Number of peaks

940 (qval < 1E-05)