Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7 cells, H3K4me3 ChIP

cell line

MCF7

cell type

Breast cancer cell line

treatment

Control

chip antibody

H3K4me3 (Abcam, ab8580, lot GR1902371-1 and GR224370-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

34736269

Reads aligned (%)

95.8

Duplicates removed (%)

35.3

Number of peaks

24915 (qval < 1E-05)

hg19

Number of total reads

34736269

Reads aligned (%)

95.6

Duplicates removed (%)

35.5

Number of peaks

24794 (qval < 1E-05)