Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
MCF10A cells, H3K4me1 ChIP
cell line
MCF10A
cell type
Breast cancer cell line
treatment
Control
chip antibody
H3K4me1 (Abcam, ab8895)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
68619856
Reads aligned (%)
94.3
Duplicates removed (%)
5.1
Number of peaks
113405 (qval < 1E-05)

hg19

Number of total reads
68619856
Reads aligned (%)
94.1
Duplicates removed (%)
5.4
Number of peaks
112945 (qval < 1E-05)

Base call quality data from DBCLS SRA