Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Breast
Cell type
76NF2V
NA
NA

Attributes by original data submitter

Sample

source_name
76NF2V cells, H3K4me3 ChIP
cell line
76NF2V
cell type
Breast cancer cell line
treatment
Control
chip antibody
H3K4me3 (Abcam, ab8580)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
79995960
Reads aligned (%)
94.6
Duplicates removed (%)
35.9
Number of peaks
43303 (qval < 1E-05)

hg19

Number of total reads
79995960
Reads aligned (%)
94.4
Duplicates removed (%)
36.1
Number of peaks
43116 (qval < 1E-05)

Base call quality data from DBCLS SRA