Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Neutrophils

MeSH Description

Immature neutrophils.

Sample

source_name

Sorted neutrophils

cell type

neutrophil

passages

ex vivo non-cultured

strain

WT (C57BL6)

chip antibody

normal rabbit IgG _200 µg/0.5 ml (Santa Cruz sc-2027)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. libraries were prepared using the KAPA Hyper Prep Kit

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

38898227

Reads aligned (%)

94.8

Duplicates removed (%)

43.9

Number of peaks

3670 (qval < 1E-05)

mm9

Number of total reads

38898227

Reads aligned (%)

94.6

Duplicates removed (%)

43.9

Number of peaks

3684 (qval < 1E-05)