Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Blood

Cell type

Neutrophils

MeSH Description

Immature neutrophils.

Sample

source_name

Sorted neutrophils

cell type

neutrophil

passages

ex vivo non-cultured

strain

WT (C57BL6)

chip antibody

Rabbit polyclonal to Histone H3 (tri methyl K36) - ChIP Grade ( Abcam ab9050)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. libraries were prepared using the KAPA Hyper Prep Kit

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

30468316

Reads aligned (%)

94.8

Duplicates removed (%)

26.9

Number of peaks

240 (qval < 1E-05)

mm9

Number of total reads

30468316

Reads aligned (%)

94.3

Duplicates removed (%)

26.7

Number of peaks

258 (qval < 1E-05)