Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Blood
Cell type
Neutrophils
MeSH Description
Immature neutrophils.

Attributes by original data submitter

Sample

source_name
Sorted neutrophils
cell type
neutrophil
passages
ex vivo non-cultured
strain
WT (C57BL6)
chip antibody
Rabbit polyclonal to Histone H3 (tri methyl K4) - ChIP Grade (Abcam ab8580)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. libraries were prepared using the KAPA Hyper Prep Kit

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
30706531
Reads aligned (%)
91.5
Duplicates removed (%)
82.0
Number of peaks
19389 (qval < 1E-05)

mm9

Number of total reads
30706531
Reads aligned (%)
91.3
Duplicates removed (%)
82.1
Number of peaks
19335 (qval < 1E-05)

Base call quality data from DBCLS SRA