Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HMGB2

Cell type

Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
IMR90 cell line
cell line
IMR90
cell type
human fibroblasts
cell condition
growing cells
chip antibody
rabbit anti-HMGB2
chip antibody vendor
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
10 ng of ChIP DNAs were extracted from growing (control) and RAS-infected senescent IMR90 cells ChIP-seq libraries were prepared from 10 ng of ChIP DNAs with NEBNExt Ultra DNA Library Prep Kit for Illumina (New England Biosciences, Ipswich, MA)

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
51685337
Reads aligned (%)
88.7
Duplicates removed (%)
8.7
Number of peaks
1423 (qval < 1E-05)

hg38

Number of total reads
51685337
Reads aligned (%)
91.2
Duplicates removed (%)
6.9
Number of peaks
1881 (qval < 1E-05)

Base call quality data from DBCLS SRA