Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA4

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549
cell line
A549
chip antibody
GATA4(Thermo fisher,MA5-15532)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
42897852
Reads aligned (%)
51.8
Duplicates removed (%)
40.8
Number of peaks
13848 (qval < 1E-05)

hg38

Number of total reads
42897852
Reads aligned (%)
52.3
Duplicates removed (%)
40.4
Number of peaks
13816 (qval < 1E-05)

Base call quality data from DBCLS SRA