Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Embryonic stem cells

cell line

CCE

passages

<15

chip antibodies

None

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclear extracts were prepared using cell extraction buffers and sonication. Specific histone-DNA complexes were isolated using Pcgf6 antibodies. Pcgf6-bound genomic DNA was extracted and amplified using an Illumina Library Prep Kit (Illumina, IP-202-1012).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

35770201

Reads aligned (%)

95.5

Duplicates removed (%)

11.5

Number of peaks

714 (qval < 1E-05)

mm9

Number of total reads

35770201

Reads aligned (%)

95.2

Duplicates removed (%)

11.5

Number of peaks

793 (qval < 1E-05)