GSM2232670: Normal IgG replicate 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
source_name
Normal BJ1
patient id
BJ1
condition
Normal
antibody
IgG
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei pellets were resuspended in Covaris with 2mL of lysis buffer 3 (10mM Tris-HCl pH 8, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, 0.5% Na-Laurylsarcosine) and sheared using a Covaris S2 sonicator (20% duty cycle, intensity 5, 200 cycles/burst) for 9 minutes per sample. 300ug of chromatin was used for each sample. Before addition of antibodies, an aliquot of each sample was reserved for input control. After reverse cross-linking and cleanup, approximately 20ng of each sample was prepared for sequencing using the ChIP-seq sample rep kit (Illumina) according to the manufacturer’s instructions.