Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mutation at binding site from ESC_WT
tissue
ESC

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was prepared from cells or embryos using the RNeasy Plus Mini or Micro kit (Qiagen) with on-column DNase digestion. RNA-seq libraries were constructed according to Illumima protocol and sequenced with the Illumina Hiseq 2000. ChIP-seq libraries were constructied by using DNA SMART™ ChIPSeq Kit from Clontech

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
24192403
Reads aligned (%)
80.0
Duplicates removed (%)
6.5
Number of peaks
680 (qval < 1E-05)

mm9

Number of total reads
24192403
Reads aligned (%)
79.8
Duplicates removed (%)
6.5
Number of peaks
660 (qval < 1E-05)

Base call quality data from DBCLS SRA