Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

Testes, input

strain

hybrid C57BL6/J and 129/SvEv

genotype

wild type

age

postnatal day 1.5

tissue

Testes

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Testis lysates were clarified from sonicated nuclei and the ARID4B-DNA complexes were isolated with the anti-ARID4B antibody (CAT#: A302-233A, LOT#: A302-233A-1, Bethyl Laboratories). Libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

32203957

Reads aligned (%)

94.6

Duplicates removed (%)

5.1

Number of peaks

587 (qval < 1E-05)

mm9

Number of total reads

32203957

Reads aligned (%)

94.5

Duplicates removed (%)

5.2

Number of peaks

535 (qval < 1E-05)