Antigen

Antigen Class

TFs and others

Antigen

Spi1

Cell type

Cell type Class

Blood

Cell type

Macrophages

MeSH Description

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Sample

source_name

Primary bone marrow-derived macrophages, IFNg for 4hrs, PU.1 ChIP

strain/background

129S6/SvEv

cell type

bone marrow-derived macrophages (7th day of differentiation)

treatment

IFNg (100 ng/ml) for 4hrs

chip antibody

anti-PU.1 (rabbit polyclonal against the N-terminus (aa. 1-100))

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

31333520

Reads aligned (%)

79.7

Duplicates removed (%)

58.8

Number of peaks

49610 (qval < 1E-05)

mm9

Number of total reads

31333520

Reads aligned (%)

79.5

Duplicates removed (%)

58.9

Number of peaks

49785 (qval < 1E-05)