Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

T-47D

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

Breast Cancer Cell

cell line

T47D

passages

5 to 10

chip-antibody

ER alpha (sc543) Santa Cruz

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were lysed and sonicated leading to a DNA average size of 200bp. Protein G dynabeads were pre-incubated with 5μg of ER alpha (sc-543) or FOXA1 (ab5089) with pre cleared chromatin. The complexes were reverse cross-linked and DNA was purified. ChIP-seq libraries were prepared using 10 ng of DNA and Illumina's TruSeq ChIP sample prep.Libraries were validated using the Agient Technologies 2100 Bioanalyzer and Qubit high sensitivity assay.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

44629288

Reads aligned (%)

91.5

Duplicates removed (%)

9.5

Number of peaks

8401 (qval < 1E-05)

hg38

Number of total reads

44629288

Reads aligned (%)

92.3

Duplicates removed (%)

8.2

Number of peaks

8332 (qval < 1E-05)