Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me2

Cell type

Cell type Class
Digestive tract
Cell type
MGC-803
NA
NA

Attributes by original data submitter

Sample

source_name
gastric cancer cells
Sex
male
tissue
stomach
cell line
MGC803 gastric cancer cell line
age
53
genotype/variation
LSD1 KO
chip antibody
anti-H3K9me2 (Abcam, catalog# ab1220, lot# GR139816-1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
22720583
Reads aligned (%)
97.8
Duplicates removed (%)
2.4
Number of peaks
1172 (qval < 1E-05)

hg19

Number of total reads
22720583
Reads aligned (%)
96.8
Duplicates removed (%)
2.9
Number of peaks
580 (qval < 1E-05)

Base call quality data from DBCLS SRA