Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me2

Cell type

Cell type Class
Digestive tract
Cell type
MGC-803
NA
NA

Attributes by original data submitter

Sample

source_name
gastric cancer cells
Sex
male
tissue
stomach
cell line
MGC803 gastric cancer cell line
age
53
genotype/variation
LSD1 KO
chip antibody
anti-H3K4me2 (Abcam, catalog# ab32356, lot# GR26908-7)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
29088538
Reads aligned (%)
97.9
Duplicates removed (%)
4.5
Number of peaks
1092 (qval < 1E-05)

hg19

Number of total reads
29088538
Reads aligned (%)
97.2
Duplicates removed (%)
4.9
Number of peaks
596 (qval < 1E-05)

Base call quality data from DBCLS SRA