Antigen

Antigen Class

Histone

Antigen

H3K9me2

Cell type

Cell type Class

Digestive tract

Cell type

MGC-803

NA

NA

Sample

source_name

gastric cancer cells

Sex

male

tissue

stomach

cell line

MGC803 gastric cancer cell line

age

53

genotype/variation

control

chip antibody

anti-H3K9me2 (Abcam, catalog# ab1220, lot# GR139816-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

23037745

Reads aligned (%)

96.3

Duplicates removed (%)

3.9

Number of peaks

1272 (qval < 1E-05)

hg19

Number of total reads

23037745

Reads aligned (%)

95.1

Duplicates removed (%)

4.5

Number of peaks

604 (qval < 1E-05)