GSM2235689: VCaP AR R1881 4h rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AR
Cell type
Cell type Class
Prostate
Cell type
VCaP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
VCaP cells
cell line
Vertebral Cancer of Prostate (VCaP)
origin
bone metastasis of prostate cancer
antibody
rabbit antiserum against AR
treatment
10nM R1881
time
4h
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were derived from sonicated (Bioruptor, Diagenode) nuclei and AR-, CTCF- and H3K9me3-DNA complexes were isolated with 1 microgram of antibody and protein-A magnetic beads. DNA fragments were purified and libraries were prepared for sequencing using standard Illumina protocol. Samples were deep-sequenced with HiSeq2000 at the GeneCore in EMBL (Heidelberg, Germany).