Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
SK-N-BE(2)
Primary Tissue
Brain
Site of Extraction
Bone Marrow
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
bone marrow metastatic neuroblastoma
cell type
neuroblastoma cell line
cell line
SK-N-BE2
chip antibody
input

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed for protein, with subsequent protein quantified by spectrophotometry. Protein was resolved on 8–14% Tris-Bis gels, transferred to PVDF membranes, blocked and subjected to primary and secondary antibodies

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
69889413
Reads aligned (%)
75.0
Duplicates removed (%)
21.6
Number of peaks
643 (qval < 1E-05)

hg19

Number of total reads
69889413
Reads aligned (%)
74.6
Duplicates removed (%)
22.1
Number of peaks
874 (qval < 1E-05)

Base call quality data from DBCLS SRA