Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Lung

Cell type

Lung

MeSH Description

Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.

Sample

source_name

Lung, H3K27me3 and H3K4me1 ChIPs

strain/background

C57BL/6

tissue

Lung

age

Adult

chip antibody1

H3K27me3 (Millipore 07-449)

chip antibody2

H3K4me1 (Abcam ab8895)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Revert crosslinking and purify ChIPed DNA with AMPure beads. High-throughput reChIP, developed from the iChIP protocol (Lara-Astiaso, Weiner et al. Science 2014 [PMID 25103404]).

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

41777318

Reads aligned (%)

96.4

Duplicates removed (%)

52.7

Number of peaks

2280 (qval < 1E-05)

mm9

Number of total reads

41777318

Reads aligned (%)

96.1

Duplicates removed (%)

52.7

Number of peaks

2221 (qval < 1E-05)