Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Kidney
Cell type
Kidney
MeSH Description
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.

Attributes by original data submitter

Sample

source_name
Kidney, H3K27me3 and H3K4me3 ChIPs
strain/background
C57BL/6
tissue
Kidney
age
Adult
chip antibody1
H3K27me3 (Millipore 07-449)
chip antibody2
H3K4me3 (Millipore 07-473)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Revert crosslinking and purify ChIPed DNA with AMPure beads. High-throughput reChIP, developed from the iChIP protocol (Lara-Astiaso, Weiner et al. Science 2014 [PMID 25103404]).

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
23912936
Reads aligned (%)
96.6
Duplicates removed (%)
55.0
Number of peaks
3778 (qval < 1E-05)

mm9

Number of total reads
23912936
Reads aligned (%)
96.4
Duplicates removed (%)
55.2
Number of peaks
3662 (qval < 1E-05)

Base call quality data from DBCLS SRA