Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Blood
Cell type
SKNO-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
SKNO1 cells
fusion protein
AML-ETO
cell line
SKNO1
cell type
AML cell line
chip antibody
H3K4me1 antibody (AbCam ab8895)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
25774997
Reads aligned (%)
95.0
Duplicates removed (%)
3.3
Number of peaks
8913 (qval < 1E-05)

hg19

Number of total reads
25774997
Reads aligned (%)
94.5
Duplicates removed (%)
4.2
Number of peaks
8893 (qval < 1E-05)

Base call quality data from DBCLS SRA