Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

SKNO-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia

Sample

source_name

SKNO1 cells

fusion protein

AML-ETO

cell line

SKNO1

cell type

AML cell line

chip antibody

H3K27me3 antibody (Millipore 07-449)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

36427551

Reads aligned (%)

88.0

Duplicates removed (%)

10.5

Number of peaks

2214 (qval < 1E-05)

hg19

Number of total reads

36427551

Reads aligned (%)

87.6

Duplicates removed (%)

11.1

Number of peaks

2260 (qval < 1E-05)