Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
hES H1
cell line
H1
chip antibody
H3K36me3, Abcam, ab9050

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed with the Millipore ChIP Assay Kit (Millipore, 17-295) Libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, E7370S) and sequenced on NextSeq500

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
84527649
Reads aligned (%)
75.8
Duplicates removed (%)
8.0
Number of peaks
96823 (qval < 1E-05)

hg19

Number of total reads
84527649
Reads aligned (%)
75.4
Duplicates removed (%)
8.2
Number of peaks
96382 (qval < 1E-05)

Base call quality data from DBCLS SRA