Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Larvae
Cell type
L3
NA
NA

Attributes by original data submitter

Sample

source_name
L3 worms
strain
N2
developmental stage
L3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The pellet was resuspended in one volume of FA buffer (50 mM HEPES/KOH pH 7.5, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate; 150 mM NaCl) plus protease and phosphatase inhibitors. To obtain chromatin fragments lysate was sonicated on high, for 30 seconds on 30 seconds off, two times for 10 minutes and one time for 15 minutes. 50 ul of the lystate was used to make IP input library. 3 ug of anti-H4K16ac (Millipore 07-329) was added to 400 ul of lysate (containing approximately 50 ug DNA) for ChIP. ChIP DNA were ligated to Illumina adaptors and amplified by PCR. Library DNA between 200-550 bp in size.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

ce11

Number of total reads
18019537
Reads aligned (%)
96.8
Duplicates removed (%)
11.8
Number of peaks
550 (qval < 1E-05)

ce10

Number of total reads
18019537
Reads aligned (%)
96.8
Duplicates removed (%)
11.8
Number of peaks
548 (qval < 1E-05)

Base call quality data from DBCLS SRA