Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Muscle

Cell type

LHCN-M2

Tissue

skeletal muscle myoblast

Lineage

mesoderm

Description

skeletal myoblasts derived from satellite cells from the pectoralis major muscle of a 41 year old caucasian heart transplant donor, immortalized with lox-hygro-hTERT ("LH"), and Cdk4-neo ("CN"), Zhu et al. (2007) in Aging Cell, vol. 6, pp 515-523, newly promoted to tier 2: not in 2011 analysis.

Sample

source_name

LHCN-M2

biomaterial_provider

Woodring Wright, M.D., Ph.D.

lab

UW

lab description

Stamatoyannopoulous - University of Washington

datatype

DnaseDgf

datatype description

DNase Digital Footprinting

cell

LHCN-M2

cell organism

human

cell description

skeletal myoblasts derived from satellite cells from the pectoralis major muscle of a 41 year old caucasian heart transplant donor, immortalized with lox-hygro-hTERT ("LH"), and Cdk4-neo ("CN"), Zhu et al. (2007) in Aging Cell, vol. 6, pp 515-523, newly promoted to tier 2: not in 2011 analysis.

cell lineage

mesoderm

cell sex

M

labexpid

DS20548

labversion

Hotspot-v5.2,lmax-v1.0,WindowDensity-bin20-win+/-75

Sequenced DNA Library

library_name

GSM1014524: UW_DnaseDgf_LHCN-M2

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

223562945

Reads aligned (%)

89.8

Duplicates removed (%)

55.9

Number of peaks

209331 (qval < 1E-05)

hg19

Number of total reads

223562945

Reads aligned (%)

89.0

Duplicates removed (%)

56.9

Number of peaks

214747 (qval < 1E-05)