Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
esg

Cell type

Cell type Class
Adult
Cell type
Intestines
NA
NA

Attributes by original data submitter

Sample

source_name
adult intestine, 3HA-Esg ChIP
genotype/variation
3HA-esg transgene
tissue
intestine
age
adult
treatment
7 days at 29°C
chip antibody
anti-HA tag antibody - ChIP grade (Abcam, ab9110)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody according to ChIP-IT® High Sensitivity kit (Active Motif). Libraries were prepared for sequencing using NEBNext Ultra DNA Library Preparation Kit for Illumina.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
7808827
Reads aligned (%)
145.6
Duplicates removed (%)
16.0
Number of peaks
1121 (qval < 1E-05)

dm6

Number of total reads
7808827
Reads aligned (%)
142.3
Duplicates removed (%)
15.7
Number of peaks
983 (qval < 1E-05)

Base call quality data from DBCLS SRA