Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
sc

Cell type

Cell type Class
Adult
Cell type
Intestines
NA
NA

Attributes by original data submitter

Sample

source_name
adult intestine, 3HA-Sc ChIP
genotype/variation
3HA-sc transgene
tissue
intestine
age
adult
treatment
3 days at 29°C
chip antibody
anti-HA tag antibody - ChIP grade (Abcam, ab9110)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody according to ChIP-IT® High Sensitivity kit (Active Motif). Libraries were prepared for sequencing using NEBNext Ultra DNA Library Preparation Kit for Illumina.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
8779876
Reads aligned (%)
165.4
Duplicates removed (%)
10.1
Number of peaks
7409 (qval < 1E-05)

dm6

Number of total reads
8779876
Reads aligned (%)
165.0
Duplicates removed (%)
10.3
Number of peaks
7833 (qval < 1E-05)

Base call quality data from DBCLS SRA