Antigen

Antigen Class

TFs and others

Antigen

sc

Cell type

Cell type Class

Adult

Cell type

Intestines

NA

NA

Sample

source_name

adult intestine, 3HA-Sc ChIP

genotype/variation

3HA-sc transgene

tissue

intestine

age

adult

treatment

3 days at 29°C

chip antibody

anti-HA tag antibody - ChIP grade (Abcam, ab9110)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody according to ChIP-IT® High Sensitivity kit (Active Motif). Libraries were prepared for sequencing using NEBNext Ultra DNA Library Preparation Kit for Illumina.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm6

Number of total reads

8779876

Reads aligned (%)

91.9

Duplicates removed (%)

10.3

Number of peaks

7833 (qval < 1E-05)

dm3

Number of total reads

8779876

Reads aligned (%)

92.0

Duplicates removed (%)

10.1

Number of peaks

7409 (qval < 1E-05)