Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Kidney

Cell type

Patski

Tissue

Kidney

Lineage

cellLine

Description

Mouse Embryonic Kidney Fibroblast. As described in Lingenfelter et al., 1998 (Nat Genet. 1998 18:212-3) and Yang et al., 2010 (Genome Res. 2010 20:614-22), PATSKI is a female interspecific mouse fibroblast that was derived from the embryonic kidney of an M.spretus x C57BL/6J hybrid mouse such that the C57Bl/6J X chromosome (maternal) is always the inactive X. This is an adherent cell line.

Sample

source_name

Patski

biomaterial_provider

Dr. Christine Disteche (University of Washington)

lab

UW-m

lab description

Stamatoyannopoulous - University of Washington

datatype

DnaseSeq

datatype description

DNaseI HS Sequencing

cell

Patski

cell organism

mouse

cell description

Mouse Embryonic Kidney Fibroblast. As described in Lingenfelter et al., 1998 (Nat Genet. 1998 18:212-3) and Yang et al., 2010 (Genome Res. 2010 20:614-22), PATSKI is a female interspecific mouse fibroblast that was derived from the embryonic kidney of an M.spretus x C57BL/6J hybrid mouse such that the C57Bl/6J X chromosome (maternal) is always the inactive X. This is an adherent cell line.

cell sex

M

age

immortalized

age description

Immortal cells

strain

Spretus.BL6-Xist

strain description

M.spretus x C57BL/6J hybrid mouse such that the C57Bl/6J X chromosome (maternal) is always the inactive X.

age

immortalized

age description

Immortal cells

labversion

1,Hotspot-v5.1,lmax-v1.1,WindowDensity-bin20-win+/-75

replicate

1,2

strain description

M.spretus x C57BL/6J hybrid mouse such that the C57Bl/6J X chromosome (maternal) is always the inactive X.

Sequenced DNA Library

library_name

GSM1014171: UW_DnaseSeq_Patski_immortalized_Spretus.BL6-Xist

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

74552490

Reads aligned (%)

91.9

Duplicates removed (%)

33.4

Number of peaks

67740 (qval < 1E-05)

mm9

Number of total reads

74552490

Reads aligned (%)

91.7

Duplicates removed (%)

33.4

Number of peaks

67791 (qval < 1E-05)