Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
E2F6

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

GEO Accession
GSM1010899
antibody antibodydescription
Rabbit polyclonal IgG, epitope corresponding to a.a. 232-281 mapping at the C-terminus of E2F-6 of human origin. Antibody Target: E2F6
antibody targetdescription
This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms (RefSeq).
antibody vendorid
sc-22823
antibody vendorname
Santa Cruz Biotechnology
antibody
E2F6
cell description
embryonic stem cells
cell karyotype
normal
cell lineage
inner cell mass
cell organism
human
cell sex
M
cell
H1-hESC
controlid
SL12796
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL13304,SL12414
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010899: HudsonAlpha_ChipSeq_H1-hESC_E2F6_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
62291189
Reads aligned (%)
82.0
Duplicates removed (%)
8.8
Number of peaks
40010 (qval < 1E-05)

hg38

Number of total reads
62291189
Reads aligned (%)
82.7
Duplicates removed (%)
8.4
Number of peaks
40121 (qval < 1E-05)

Base call quality data from DBCLS SRA