Antigen

Antigen Class

TFs and others

Antigen

MAX

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H1

NA

NA

Sample

GEO Accession

GSM1010898

antibody antibodydescription

Rabbit polyclonal IgG, epitope mapping at the C-terminus of Max of human origin. Antibody Target: Max

antibody targetdescription

The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown (RefSeq).

antibody vendorid

sc-197

antibody vendorname

Santa Cruz Biotechnology

antibody

Max

cell description

embryonic stem cells

cell karyotype

normal

cell lineage

inner cell mass

cell organism

human

cell sex

M

cell

H1-hESC

controlid

SL12796

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL12411,SL13302

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010898: HudsonAlpha_ChipSeq_H1-hESC_Max_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

60115116

Reads aligned (%)

95.3

Duplicates removed (%)

5.6

Number of peaks

71635 (qval < 1E-05)

hg19

Number of total reads

60115116

Reads aligned (%)

95.0

Duplicates removed (%)

6.0

Number of peaks

71414 (qval < 1E-05)