Antigen

Antigen Class

TFs and others

Antigen

CREB1

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

GEO Accession

GSM1010890

antibody antibodydescription

Mouse monoclonal IgG1; raised against amino acids 254-327 mapping within the DNA binding and dimerization domain of human CREB-1. Antibody Target: CREB1

antibody targetdescription

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)

antibody vendorid

sc-240

antibody vendorname

Santa Cruz Biotechnology

antibody

CREB1_(SC-240)

cell description

leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC

cell karyotype

cancer

cell lineage

mesoderm

cell organism

human

cell sex

F

cell

K562

controlid

SL12793

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL13293,SL12420

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010890: HudsonAlpha_ChipSeq_K562_CREB1_(SC-240)_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

74676242

Reads aligned (%)

87.5

Duplicates removed (%)

9.1

Number of peaks

20231 (qval < 1E-05)

hg19

Number of total reads

74676242

Reads aligned (%)

87.1

Duplicates removed (%)

9.7

Number of peaks

20864 (qval < 1E-05)