Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CEBPB

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

GEO Accession
GSM1010889
antibody antibodydescription
Rabbit polyclonal IgG, epitope mapping at the C-terminus of CEBPB of rat origin. Antibody Target: CEBPB
antibody targetdescription
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene (provided by RefSeq).
antibody vendorid
sc-150
antibody vendorname
Santa Cruz Biotechnology
antibody
CEBPB_(SC-150)
cell description
mammary gland, adenocarcinoma. (PMID: 4357757), newly promoted to tier 2: not in 2011 analysis
cell karyotype
cancer
cell lineage
ectoderm
cell organism
human
cell sex
F
cell
MCF-7
controlid
SL13504
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL13460,SL14684
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010889: HudsonAlpha_ChipSeq_MCF-7_CEBPB_(SC-150)_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
50157081
Reads aligned (%)
86.0
Duplicates removed (%)
8.9
Number of peaks
52375 (qval < 1E-05)

hg38

Number of total reads
50157081
Reads aligned (%)
87.4
Duplicates removed (%)
7.9
Number of peaks
52521 (qval < 1E-05)

Base call quality data from DBCLS SRA