Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EGR1

Cell type

Cell type Class
Uterus
Cell type
ECC-1
Primary Tissue
Uterus
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

GEO Accession
GSM1010888
antibody antibodydescription
Rabbit polyclonal, EGR-1 (588). Antibody Target: EGR1
antibody targetdescription
Egr-1, Egr-2, Egr-3 and Egr-4 are nuclear transcription factors belonging to the Egr C2H2-type zinc-finger protein family and containing three C2H2-type zinc fingers. As immediate early proteins, Egr transcription factors are rapidly induced by diverse extracellular stimuli. Egr proteins are subject to tight differential control through diverse mechanisms at several levels of regulation including transcriptional, translational and post-translational (including glyco- sylation, phosphorylation and redox) mechanisms and protein-protein inter- action. Egr-1 binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site), there- by activating transcription of target genes whose products are required for mitogenisis and differentiation. Egr-2 binds specific DNA sites located in the promoter region of HoxA4. Egr-2 defects cause congenital hypomyelination neuropathy (also designated Charcot-Marie-tooth disease) and Dejerine- Sottas neuropathology (also designated hereditary motor and sensory neuro- pathy III. Egr-3 is involved in muscle spindle development and is expressed in T cells 20 minutes following activation. EGR-4 binds to the EGR consensus motif GCGTGGGCG, functions as a transcriptional repressor, and displays autoregulatory activities, downregulating its on gene promoter in a dose dependent manner.
antibody vendorid
sc-110
antibody vendorname
Santa Cruz Biotechnology
antibody
Egr-1
cell description
epithelial cell line derived from an endometrium adenocarcinoma, fetal membranes were collected from women who underwent planned cesarean delivery at term, before labor and without rupture of membranes
cell karyotype
cancer
cell lineage
endoderm
cell organism
human
cell sex
F
cell
ECC-1
controlid
SL13506
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL14590,SL13280
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
ERROR: cannot retrieve data from NCBI: too many requests
library_strategy
ERROR: cannot retrieve data from NCBI: too many requests
library_source
ERROR: cannot retrieve data from NCBI: too many requests
library_selection
ERROR: cannot retrieve data from NCBI: too many requests
library_construction_protocol
ERROR: cannot retrieve data from NCBI: too many requests

Sequencing Platform

instrument_model
ERROR: cannot retrieve data from NCBI: too many requests
cycle_sequence
ERROR: cannot retrieve data from NCBI: too many requests
cycle_count
ERROR: cannot retrieve data from NCBI: too many requests
flow_sequence
ERROR: cannot retrieve data from NCBI: too many requests
flow_count
ERROR: cannot retrieve data from NCBI: too many requests
key_sequence
ERROR: cannot retrieve data from NCBI: too many requests

hg19

Number of total reads
61928846
Reads aligned (%)
84.5
Duplicates removed (%)
6.0
Number of peaks
11539 (qval < 1E-05)

hg38

Number of total reads
61928846
Reads aligned (%)
85.7
Duplicates removed (%)
5.2
Number of peaks
11583 (qval < 1E-05)

Base call quality data from DBCLS SRA