Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
STAT5A

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

GEO Accession
GSM1010877
antibody antibodydescription
Mouse monoclonal IgG, recommended for the detection of Stat5a and Stat5b of human origin by WB, IP, IF and ELISA. Antibody Target: STAT5A
antibody targetdescription
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. (provided by RefSeq)
antibody vendorid
sc-74442
antibody vendorname
Santa Cruz Biotechnology
antibody
STAT5A_(SC-74442)
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell organism
human
cell sex
F
cell
K562
controlid
SL6023,SL9103
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL9116,SL8673
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010877: HudsonAlpha_ChipSeq_K562_STAT5A_(SC-74442)_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
99327005
Reads aligned (%)
81.1
Duplicates removed (%)
5.8
Number of peaks
17783 (qval < 1E-05)

hg38

Number of total reads
99327005
Reads aligned (%)
82.4
Duplicates removed (%)
4.8
Number of peaks
17768 (qval < 1E-05)

Base call quality data from DBCLS SRA