Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYBL2

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

GEO Accession
GSM1010876
antibody antibodydescription
Mouse monoclonal IgG, raised against a recombinant protein corresponding to an internal region of B-Myb of human origin. Antibody Target: MYBL2
antibody targetdescription
The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined. (provided by RefSeq)
antibody vendorid
sc-81192
antibody vendorname
Santa Cruz Biotechnology
antibody
MYBL2_(SC-81192)
cell description
hepatocellular carcinoma
cell karyotype
cancer
cell lineage
endoderm
cell organism
human
cell sex
M
cell
HepG2
controlid
SL6019
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL7564,SL7101
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010876: HudsonAlpha_ChipSeq_HepG2_MYBL2_(SC-81192)_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
80021279
Reads aligned (%)
78.7
Duplicates removed (%)
9.5
Number of peaks
32847 (qval < 1E-05)

hg38

Number of total reads
80021279
Reads aligned (%)
79.9
Duplicates removed (%)
8.7
Number of peaks
32685 (qval < 1E-05)

Base call quality data from DBCLS SRA