Antigen

Antigen Class

TFs and others

Antigen

REST

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

GEO Accession

GSM1010869

antibody antibodydescription

Mouse monoclonal. Antibody Target: NRSF

antibody targetdescription

Neuron-restrictive silencer transcription factor

antibody vendorid

(Made by Protein Expression Center: pec@caltech.edu)

antibody vendorname

David Anderson, Caltech

antibody

NRSF

cell description

colorectal carcinoma (PMID: 7214343)

cell karyotype

cancer

cell lineage

endoderm

cell organism

human

cell sex

M

cell

HCT-116

controlid

SL12247,SL12246

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL16310,SL14560

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010869: HudsonAlpha_ChipSeq_HCT-116_NRSF_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg19

Number of total reads

51917426

Reads aligned (%)

87.4

Duplicates removed (%)

7.5

Number of peaks

6321 (qval < 1E-05)

hg38

Number of total reads

51917426

Reads aligned (%)

88.4

Duplicates removed (%)

6.8

Number of peaks

6282 (qval < 1E-05)