Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFIC

Cell type

Cell type Class
Uterus
Cell type
ECC-1
Primary Tissue
Uterus
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

GEO Accession
GSM1010855
antibody antibodydescription
Mouse monoclonal IgG, raised against a recombinant protein corresponding to an internal region of NF-1 of human origin. Antibody Target: NFIC
antibody targetdescription
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
antibody vendorid
sc-81335
antibody vendorname
Santa Cruz Biotechnology
antibody
NFIC_(SC-81335)
cell description
epithelial cell line derived from an endometrium adenocarcinoma, fetal membranes were collected from women who underwent planned cesarean delivery at term, before labor and without rupture of membranes
cell karyotype
cancer
cell lineage
endoderm
cell organism
human
cell sex
F
cell
ECC-1
controlid
SL16754,SL13506
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL14056,SL16022
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010855: HudsonAlpha_ChipSeq_ECC-1_NFIC_(SC-81335)_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
79738439
Reads aligned (%)
84.4
Duplicates removed (%)
8.7
Number of peaks
45902 (qval < 1E-05)

hg38

Number of total reads
79738439
Reads aligned (%)
85.4
Duplicates removed (%)
8.1
Number of peaks
46014 (qval < 1E-05)

Base call quality data from DBCLS SRA