Antigen

Antigen Class

TFs and others

Antigen

CEBPB

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

GEO Accession

GSM1010852

antibody antibodydescription

Rabbit polyclonal IgG, epitope mapping at the C-terminus of CEBPB of rat origin. Antibody Target: CEBPB

antibody targetdescription

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene (provided by RefSeq).

antibody vendorid

sc-150

antibody vendorname

Santa Cruz Biotechnology

antibody

CEBPB_(SC-150)

cell description

colorectal carcinoma (PMID: 7214343)

cell karyotype

cancer

cell lineage

endoderm

cell organism

human

cell sex

M

cell

HCT-116

controlid

SL12247,SL12246

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL12240,SL13073

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010852: HudsonAlpha_ChipSeq_HCT-116_CEBPB_(SC-150)_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

57295571

Reads aligned (%)

90.6

Duplicates removed (%)

7.8

Number of peaks

40972 (qval < 1E-05)

hg19

Number of total reads

57295571

Reads aligned (%)

90.2

Duplicates removed (%)

8.4

Number of peaks

40962 (qval < 1E-05)