Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TCF12

Cell type

Cell type Class
Uterus
Cell type
ECC-1
Primary Tissue
Uterus
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

GEO Accession
GSM1010842
antibody antibodydescription
HEB (A-20) Rabbit polyclonal IgG, 200 ?g/ml. Epitope mapping within the C-terminus of HEB of human origin. Antibody Target: TCF12
antibody targetdescription
The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
antibody vendorid
sc-357
antibody vendorname
Santa Cruz Biotechnology
antibody
TCF12
cell description
epithelial cell line derived from an endometrium adenocarcinoma, fetal membranes were collected from women who underwent planned cesarean delivery at term, before labor and without rupture of membranes
cell karyotype
cancer
cell lineage
endoderm
cell organism
human
cell sex
F
cell
ECC-1
controlid
SL16754,SL13506
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL16025,SL14063
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010842: HudsonAlpha_ChipSeq_ECC-1_TCF12_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
55356153
Reads aligned (%)
79.9
Duplicates removed (%)
9.1
Number of peaks
30156 (qval < 1E-05)

hg38

Number of total reads
55356153
Reads aligned (%)
80.9
Duplicates removed (%)
8.4
Number of peaks
30259 (qval < 1E-05)

Base call quality data from DBCLS SRA