Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

GEO Accession
GSM1010830
antibody antibodydescription
mouse monoclonal, clone 8WG16. Antibody Target: POL2
antibody targetdescription
RNA Polymerase II
antibody vendorid
MMS-126R
antibody vendorname
Covance
antibody
Pol2
cell description
umbilical vein endothelial cells
cell karyotype
normal
cell lineage
mesoderm
cell organism
human
cell sex
U
cell
HUVEC
controlid
SL1106
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL6966,SL1103
protocol description
one 15-cycle round of PCR (Myers)
protocol
PCR1x
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010830: HudsonAlpha_ChipSeq_HUVEC_Pol2_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
61903954
Reads aligned (%)
86.8
Duplicates removed (%)
10.4
Number of peaks
29288 (qval < 1E-05)

hg19

Number of total reads
61903954
Reads aligned (%)
86.2
Duplicates removed (%)
10.6
Number of peaks
30192 (qval < 1E-05)

Base call quality data from DBCLS SRA