Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

GEO Accession
GSM1010823
antibody antibodydescription
Rabbit affinity purified polyclonal antibody. Epitope is the C-terminus of human estrogen receptor alpha (ESR1). Recommended for detection of estrogen receptor of m, r and h origin by WB, IP, IF, IHC(P) and ELISA. Antibody Target: ESR1
antibody targetdescription
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5-prime UTRs and use different promoters. (provided by RefSeq)
antibody vendorid
sc-543
antibody vendorname
Santa Cruz Biotechnology
antibody
ERalpha_a
cell description
epithelial cell line derived from a mammary ductal carcinoma.
cell karyotype
cancer
cell organism
human
cell sex
F
cell
T-47D
controlid
SL1490
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL1877,SL1493
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
protocol
v041610.2
replicate
1,2
softwareversion
MACS
treatment description
1 h with 100 nM Bisphenol A (Myers)
treatment
BPA_100nM

Sequenced DNA Library

library_name
GSM1010823: HudsonAlpha_ChipSeq_T-47D_ERalpha_a_BPA_100nM_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
70820678
Reads aligned (%)
74.1
Duplicates removed (%)
14.5
Number of peaks
7134 (qval < 1E-05)

hg38

Number of total reads
70820678
Reads aligned (%)
76.6
Duplicates removed (%)
12.4
Number of peaks
7183 (qval < 1E-05)

Base call quality data from DBCLS SRA