Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR2F2

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

GEO Accession
GSM1010810
antibody antibodydescription
Mouse monoclonal IgG, specific for an epitope mapping between amino acids 13-44 at the N-terminus of NR2F2 of human origin. Antibody Target: NR2F2
antibody targetdescription
This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. (provided by RefSeq)
antibody vendorid
sc-271940
antibody vendorname
Santa Cruz Biotechnology
antibody
NR2F2_(SC-271940)
cell description
hepatocellular carcinoma
cell karyotype
cancer
cell lineage
endoderm
cell organism
human
cell sex
M
cell
HepG2
controlid
SL12050,SL12795
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL12644,SL16316
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010810: HudsonAlpha_ChipSeq_HepG2_NR2F2_(SC-271940)_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
54168949
Reads aligned (%)
83.2
Duplicates removed (%)
8.5
Number of peaks
37197 (qval < 1E-05)

hg38

Number of total reads
54168949
Reads aligned (%)
84.1
Duplicates removed (%)
7.9
Number of peaks
36722 (qval < 1E-05)

Base call quality data from DBCLS SRA