Antigen

Antigen Class

TFs and others

Antigen

REST

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC derived neural cells

NA

NA

Sample

GEO Accession

GSM1010804

antibody antibodydescription

Mouse monoclonal. Antibody Target: NRSF

antibody targetdescription

Neuron-restrictive silencer transcription factor

antibody vendorid

(Made by Protein Expression Center: pec@caltech.edu)

antibody vendorname

David Anderson, Caltech

antibody

NRSF

cell description

neurons derived from H1 embryonic stem cells, newly promoted to tier 2: not in 2011 analysis

cell karyotype

normal

cell lineage

ectoderm

cell organism

human

cell sex

M

cell

H1-neurons

controlid

SL13320,SL16755

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL13299,SL14593

protocol description

Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)

protocol

v042211.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010804: HudsonAlpha_ChipSeq_H1-neurons_NRSF_v042211.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

68209566

Reads aligned (%)

92.5

Duplicates removed (%)

7.2

Number of peaks

39421 (qval < 1E-05)

hg19

Number of total reads

68209566

Reads aligned (%)

92.0

Duplicates removed (%)

8.1

Number of peaks

39188 (qval < 1E-05)