Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
REST

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived neural cells
NA
NA

Attributes by original data submitter

Sample

GEO Accession
GSM1010804
antibody antibodydescription
Mouse monoclonal. Antibody Target: NRSF
antibody targetdescription
Neuron-restrictive silencer transcription factor
antibody vendorid
(Made by Protein Expression Center: pec@caltech.edu)
antibody vendorname
David Anderson, Caltech
antibody
NRSF
cell description
neurons derived from H1 embryonic stem cells, newly promoted to tier 2: not in 2011 analysis
cell karyotype
normal
cell lineage
ectoderm
cell organism
human
cell sex
M
cell
H1-neurons
controlid
SL13320,SL16755
datatype description
Chromatin IP Sequencing
datatype
ChipSeq
lab description
Myers - Hudson Alpha Institute for Biotechnology
lab
HudsonAlpha
labexpid
SL13299,SL14593
protocol description
Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
protocol
v042211.1
replicate
1,2
softwareversion
MACS
treatment description
No special treatment or protocol applies
treatment
None

Sequenced DNA Library

library_name
GSM1010804: HudsonAlpha_ChipSeq_H1-neurons_NRSF_v042211.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
68209566
Reads aligned (%)
84.6
Duplicates removed (%)
8.1
Number of peaks
39188 (qval < 1E-05)

hg38

Number of total reads
68209566
Reads aligned (%)
85.9
Duplicates removed (%)
7.2
Number of peaks
39421 (qval < 1E-05)

Base call quality data from DBCLS SRA