Antigen

Antigen Class

TFs and others

Antigen

REST

Cell type

Cell type Class

Neural

Cell type

PFSK-1

Primary Tissue

Brain

Tissue Diagnosis

Medulloblastoma

Sample

GEO Accession

GSM1010786

antibody antibodydescription

Mouse monoclonal. Antibody Target: NRSF

antibody targetdescription

Neuron-restrictive silencer transcription factor

antibody vendorid

(Made by Protein Expression Center: pec@caltech.edu)

antibody vendorname

David Anderson, Caltech

antibody

NRSF

cell description

neuroectodermal cell line derived from a cerebral brain tumor, (PMID: 1316433)

cell karyotype

cancer

cell lineage

ectoderm

cell organism

human

cell sex

M

cell

PFSK-1

controlid

SL2339

datatype description

Chromatin IP Sequencing

datatype

ChipSeq

lab description

Myers - Hudson Alpha Institute for Biotechnology

lab

HudsonAlpha

labexpid

SL5497,SL5496

protocol description

2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)

protocol

v041610.1

replicate

1,2

softwareversion

MACS

treatment description

No special treatment or protocol applies

treatment

None

Sequenced DNA Library

library_name

GSM1010786: HudsonAlpha_ChipSeq_PFSK-1_NRSF_v041610.1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg19

Number of total reads

55820590

Reads aligned (%)

71.3

Duplicates removed (%)

21.5

Number of peaks

26358 (qval < 1E-05)

hg38

Number of total reads

55820590

Reads aligned (%)

72.2

Duplicates removed (%)

20.9

Number of peaks

26409 (qval < 1E-05)